Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions:ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution in conjunction with biotinylated anti-human sRANKL (R1076-05H) as a detection antibody, allows the detection of at least 2000-4000pg/well of recombinant human sRANKL
Western Blot: 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human sRANKL is 1.5-3ng/lane, under either reducing or non-reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. Endotoxin: <1EU/ug
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
Specificity：Recognizes human sRANKL.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human sRANKL derived from E. coli.
