Applications:Suitable for use in ELISA, Western Blot, Neutralization and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Neutralization: 1.75-3ug/ml will yield one-half maximal inhibition [ND50] of the biological activity of human Macrophage Inflammatory Protein 1 beta (50ng/ml)
ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution.
In conjunction with biotinylated anti-human Macrophage Inflammatory Protein 1 beta (M1202-31D) as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human Macrophage Inflammatory Protein 1 beta
Western Blot: 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human Macrophage Inflammatory Protein 1 beta is 1.5-3ng/lane, under either reducing or non-reducing conditions
Immunohistochemistry (FFPE): 1ug/ml stains human pancreas adennocarcinoma when incubated for 2hrs ar RT.
An HRP-labeled polymer detection system was used with a DAB chromogen.
Heat induced antigen retrival with sodium citrate buffer, pH 6, is recommended
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from sera.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Specificity：Recognizes human Macrophage Inflammatory Protein 1 beta.
Isotype：IgG
Calc Applications Abbrev：E IHC N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Macrophage Inflammatory Protein 1 beta derived from E. coli.
