Interleukin-6 (IL-6) is a pleotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses.
IL-6 is produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes.
It has diverse biological functions.
It stimulated B cell differentiation and antibody production.
It synergizes with IL-3 in megakaryocyte development and platelet production.
It induces expression of hepatic acute phase proteins.
It regulated bone metabolism.
IL-6 signals through the IL-6 receptor system that consists of two chains: IL-6Ra and gp130.
Applications:Suitable for use in ELISA, Western Blot and Neutralization.
Other applications not tested.
Recommended Dilutions:Direct ELISA: ≥0.5ug/ml using 100ul/well antibody solution.
Allows the detection of 0.2-0.4ng/well of recombinant human IL-6
Western Blot: 0.1-0.2ug/ml.
The detection limit for recombinant human IL-6 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human IL-6 (1ng/ml), a concentration of 0.2-0.4ng/ml is required
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2.
Specificity：Recognizes human IL-6.
Isotype：IgG
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Hu
Immunogen：Highly pure (> 98%) recombinant human IL-6
