The detection limit is 0.2-0.4ng/well for recombinant human Interleukin 2 when used with the compatible secondary reagents
Western Blot: 0.1-0.2ug/ml detects human Interleukin 2.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human Interleukin 2 is 1.5-3ng/lane, under either reducing or non-reducing conditions
Neutralization: 0.26-0.4ug/ml yields one-half maximal inhibition [ND50] of the biological activity of human Interleukin 2 (0.8ng/ml)Immunohistochemistry (FFPE): 0.5ug/ml stains formalin-fixed, paraffin-embeddded sections of human breast invasive ductal carcinoma with an overnight incubation at 4ºC.
Heat induced antigen retrieval with sodium citrate, pH 6.0 is recommended.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Specificity：Recognizes human Interleukin 2
Isotype：IgG
Calc Applications Abbrev：E IHC N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human Interleukin 2 expressed in E. coli
