Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution in conjunction with biotinylated anti-human gAcrp30 (G1016-01H) as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human gAcrp30
Western Blot: 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human gAcrp30 is 1.5-3ng/lane, under either reducing or non-reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
Specificity：Recognizes human gAcrp30/Adipolean.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human gAcrp30 derived from E. coli.
