Follistatin is a single chain gonadal protein that inhibits Follicle-stimulating hormone release.
It binds to and antagonizes Activin, which enhances FSH secretion.
There are three known isoforms created by alternative splicing
Follistatin is involved in the development of the embryo.
It has inhibitory action on bone morphogenic proteins (BMPs); BMPs induce the ectoderm to become epidermal ectoderm.
Inhibition of BMPs allows neuroectoderm to arise from ectoderm, a process which eventually forms the neural plate.
Other inhibitors involved in this process are noggin and chordin.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA (Direct): 1ug/ml using 100ul/well.
The detection range is 0.2-0.4ng/well of recombinant human Follistatin
ELISA (Sandwich): 0.25-1ug/ml using 100ul/well.
When this antibody is used along with F9060-01 as the capture antibody, the detection range is0.2-0.4ng/well of recombinant human Follistatin
Western Blot: 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hFollistatin is 1.5-3.0ng/lane, under either reducing or non-reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Country of Origin:USA
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with 1ml sterile PBS, 0.1% BSA.
Specificity：Recognizes human Follistatin
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Highly pure recombinant human Follistatin, expressed in E. coli
