84-1087-59　Bone Morphogenetic Protein 2 (BMP2) 50ug　B2553-07C

特徴

• Human Bone Morphogenetic Protein 2 (BMP2) is a disulfide-bonded homodimeric protein with an apparent molecular weight of 26kD.
• BMP2 regulates similiarly to its nearest homologue BMP-4 diverse fundamental processes during embryonic development: BMP2 and other BMP proteins have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions.
• BMP2 lacks the natural N-terminus which results in a 15-20 fold increase of specific activity.
• BMP2 is a heparin binding protein.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization.
• Other applications not tested.
• Recommended Dilution:ELISA (Sandwich): 0.5-2ug/ml (100ul/well); Used in conjunction with 208604 BMP-2 (Biotin) detects 0.2-0.4ng/well of recombinant hBMP-2 Western Blot: 0.1-0.2ug/ml; Detection limit is 1.5-3.0ng/lane for recombinant hBMP-2 under either reducing or non-reducing conditions
• Neutralization: 1.3-1.9ug/ml; Yields one-half maximal inhibition [ND50] of the biological activity of hBMP-2 (200ng/ml)
• Immunohistochemistry: 0.25ug/ml Formalin fixed paraffin embedded human colon/rectum adenocarcinoma with an overnight incubation at 4°C Requires heat induced antigen retrieval with a pH 6.0 sodium citrate buffer.
• An HRP-labeled polymer detection system was used with a DAB chromogen.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.