Members in the TNF superfamily regulate immune responses and induce apoptosis.
A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF-and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-kB and c-jun N-terminal Kinase).
BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion.
Two receptors for BAFF were recently identified and designated TACI and BCMA.
BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases.
Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N-terminal kinase (JNK) and to induce apoptosis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Direct ELISA: 1ug/ml.
Using 100ul/well allows the detection of 0.2-0.4ng/well of recombinant human BAFF using 208600 as the capture
Sandwich ELISA: 0.25-1ug/ml.
Using 100ul/well allows the detection of 0.2-0.4ng/well of recombinant human BAFF
Western Blot: 0.1-0.2ug/ml.
The detection limit for recombinant human BAFF is 1.5-3.0ng/lane, under either reducing or non-reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：25ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA to ~0.1-1mg/ml.
Specificity：Recognizes human BAFF.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human BAFF, expressed in E. coli.
