Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-2ug/ml.
Using 100ul/well antibody solution.
Detects 2000-4000pg/well of recombinant human Apo E3.
Western Blot: 0.1-0.2ug/ml.
The detection limit for recombinant human Apo E3 is 1.5-3ng/lane, under either reducing or non-reducing conditionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O .
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Country of Origin:USA
仕様
Size:50ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography. Endotoxin: <1EU/ug
Form:Supplied as a lyophilized powder from PBS. Reconstitute with 100ul sterile ddH2O
Specificity:Recognizes human Apolipoprotein E3 at ~150kD.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human Apolipoprotein E3, expressed in E. coli