84-1087-46　Villin 1 100ul　V2121-95

特徴

• Villin is a member of the gelsolin family of calcium-regulated actin-binding proteins.
• Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts.
• It is localized to the apical cytoplasm and brush borders of these cells.
• Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli.
• Phosphorylation of villin at Tyr60, 81 and 256 may be involved in the regulation of cell migration.
• Expression of villin is increased in colorectal cancers, and villin 1 function appears to be involved in progressive cholestasis and hepatic failure.
• Applications:Suitable for use in Western Blot and Immunofluorescence/Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunofluorescence (IC): 1:100Western Blot: 1:1000.
• Incubate membrane with diluted antibody in 1X TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.