Protein ubiquitinylation requires the concerted action of the E1, E2 and E3 ubiquitin-conjugating enzymes.
Ubiquitin is first activated through an ATP-dependent formation of a thiol ester with E1.
The activated ubiquitin is then transferred to a thiol-group of ubiquitin-conjugation enzyme E2.
The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiuitin-conjugation enzyme E3 (1)
E2-25K (Hip2) is a member of the E2 protein family that catalyzes multiubiquitin chain synthesis via Lys48 of ubiquitin (2).
E2-25K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-beta, huntingtin, and MHC-heavy chain proteins (3-5).
Lys14 of E2-25K can be modified by SUMOylation, with this modification resulting in inhibited E2 activity (6).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of total human E2-25K/Hip2 protein. Species Crossreactivity: mouse, monkey and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Arg11 of human E2-25K/Hip2 protein.
