Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1, -2 and -3) are members of the ubiquitin-like protein family (1).
The covalent attachment of the SUMO-1, -2 or -3 (sumoylation) to target proteins is analogous to ubiquitination.
This post-translational modification is a reversible, multi-step process and is initiated by cleaving a precursor protein to a mature protein.
Mature SUMO-1, -2 or -3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1, -2 or -3 is ligated to the target protein (2).
Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions.
Ubiquitin regulates predominantly degradation of its target (1).
In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and I B- to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability (3-7).
SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP and regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer's disease (8-11).
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry.
Antibody diluent: TBST/5% normal goat serumImmunofluorescence (IF-IC): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects recombinant SUMO-1 and and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP, 90kD). Species Crossreactivity: Human, mouse, monkey and rat.
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide derived from a sequence within human SUMO-1 that does not correspond to SUMO-2/3.
