Ribosomal protein L7a is a highly conserved ribosome protein localized to 60S ribosomal subunit (1).
The protein has distinct domains that target the newly synthesized polypeptide to nucleus and the nucleoli, the site of ribosome biosynthesis (2).
Ribosomal protein L7a can also interact with RNA in vitro through two distinct RNA-binding domains in the protein (3).
Taken together, nucleolar localization and the ability to bind RNA suggests that ribosomal protein L7a may act as an important component for ribosome biosynthesis and function.
Applications:Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunofluorescence (Immunocytochemistry): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Detects endogenous levels of total human ribosomal protein L7a. Immunofluorescent analysis using this antibody shows exclusive localization of ribosomal protein L7a to nucleoli during ribosomal biosynthesis. Species Crossreactivity: Monkey, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Glu109 of human ribosomal protein L7a.
