The DNA repair protein Rad50 is a member of the structural maintenance of chromosomes family (SMC) and plays an important role in cell cycle checkpoint signaling and double-strand break repair in response to DNA damage (1–4).
Rad50 forms a complex with Mre11 and Nbs1 that becomes activated in response to DNA damage (3).
In normal human cells, the MRN complex acts to tether linear DNA molecules, providing a flexible link between DNA ends (1).
Genomic instability and cancer have been shown to develop in cells with genetic mutations affecting the proteins in the MRN complex (2).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Rad50 protein. Species Crossreactivity: monkey
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to residues surrounding Lys713 of human Rad50.
