Pyruvate kinase, a glycolytic enzyme, catalyzes the conversion of phosphoenolpyruvate to pyruvate.
In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1).
The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (1).
Studies found that cancer cells exclusively express PKM2 (1-3).
PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect) (1).
When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells (1).
These cells also show decreased tumorigenicity in mouse xenografts (1).
Recent studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active tetrameric PKM2 (4).
A PKM2 mutant found in cancer cells, in which Tyr105 is replaced by phenylalanine, leads to reduced cell proliferation in hypoxia and tumor growth in xenografts in nude mice (4).
These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth (4).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human PKM2 protein at ~60kD. Species Crossreactivity: monkey, mouse and rat. Will not cross-react with PKM1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to the sequence within residues 380-440 of human PKM2. Molecular Weight: 60kD.
