84-1087-33　Proteasome alpha 6 (PSMA6) 100ul　P9076-08

特徴

• The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation.
• It consists of four stacked rings, each with 7 distinct subunits.
• The two outer layers are identical rings composed of α subunits (called PSMAs), and the two inner layers are identical rings composed of β subunits.
• While the catalytic sites are located on the β rings (1-3), the α subunits are important for assembly and as binding sites for regulatory proteins (4).
• Seven different α and ten different β proteasome genes have been identified in mammals (5).PA700, PA28 and PA200 are three major protein complexes that function as activators of the 20S proteasome.
• PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome which preferentially degrades poly-ubiquitinylated proteins (1-3).
• The proteasome has a broad substrate spectrum which includes cell cycle regulators, signaling molecules, tumor suppressors and transcription factors.
• By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune response, protein folding and disease progression (6-9).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.