Protein arginine N-methyltransferase 1 (PRMT1) is a member of the protein arginine N-methyltransferase (PRMT) family of proteins, which catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (1).
There are two types of PRMT proteins.
While both types catalyze the formation of mono-methyl arginine, Type I PRMTs (PRMT1, 3, 4, and 6) add an additional methyl group to produce asymmetric di-methyl arginine and Type II PRMTs (PRMT 5 and 7) produce symmetric di-methyl arginine (1).
Mono-methyl arginine, but not di-methyl arginine, can be converted to citrulline through deimination performed by enzymes such as PADI4 (2).
Most of the PRMTs, including PRMT1, methylate arginine residues found within glycine-arginine rich (GAR) domains of proteins, such as RGG, RG, and RXR repeats (1).
However, PRMT4/CARM1 and PRMT5 instead methylate arginine residues within PGM (proline-, glycine-, methionine-rich) motifs (3).
PRMT1 methylates Arg3 of histone H4 and cooperates synergistically with p300/CBP to enhance transcriptional activation by nuclear receptor proteins (4,5,6).
In addition, PRMT1 methylates many non-histone proteins, including the orphan nuclear receptor HNF4 (6), components of the heterogeneous nuclear ribonucleoprotein (hnRNP) particle (7), the RNA binding protein Sam68 (8), interleukin enhancer-binding factor 3 (ILF3) (9) and interferon-alpha and beta receptors (10), suggesting additional functions in transcriptional regulation, mRNA processing and signal transduction.
Alternative mRNA splicing results in three enzymatically active isoforms of PMRT1 protein that differ in their amino terminal regions (11).
PRMT1 is localized in the nucleus or cytoplasm, depending on cell type (12,13) and appears in many distinct protein complexes.
ILF3, TIS21 and the leukemia-associated BTG1 proteins bind to PRMT1 and regulate its methyltransferase activity (9,14).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptdie affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Detects endogenous levels of total PRMT1 protein (all three isoforms). Does not crossreact with other PRMT proteins. Species Crossreactivity: Human, mouse, monkey and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminus of human PRMT1. Species Sequence Homology: Bovine (100%.)
