Peroxisome proliferator-activated receptor, gamma (PPARgamma) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1).
PPARg is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2).
Besides its role in mediating adipogenesis and lipid metabolism (2), PPARg also modulates insulin sensitivity, cell proliferation and inflammation (3).
PPAR gamma transcriptional activity is inhibited by MAP kinase phosphorylation of PPAR gamma at Ser84 (4,5).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human PPAR gamma protein at 53kD (PPARg1) and 57kD (PParg2). Species crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the sequence around Asp69 of human PPAR gamma. Species sequence homology: rat.
