Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol- 4-phosphate (PIP) and phosphatidylinositol-4,5- bisphosphate (PIP2).
Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration and cell survival (1).
PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2).
PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit.
Various isoforms of the catalytic subunit (p110 alpha, p110 beta, p110 delta and p110 gamma) have been isolated, and the regulatory subunits that associate with p110 alpha, p110 beta and p110 delta are p85 alpha and p85 beta (3).
In contrast, p110 gamma associates with a p101 regulatory subunit that is unrelated to p85.
Furthermore, p110 gamma is activated by beta gamma subunits of heterotrimeric G proteins (4).
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human PI3K p110 alpha protein at 110kD. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from the human sequence of PI3K p110 alpha (KLH). Species sequence homology: bovine.
