84-1087-22　PDI (Protein Disulfide Isomerase) 100ul　P3122-11

特徴

• Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded.
• To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds.
• This process is called oxidative protein folding.
• Disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds.
• Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange.
• This event is then followed by PDI-catalyzed disulfide bond formation on folding proteins.
• Applications:Suitable for use in Western Blot, Immunofluorescence/ Immunocytochemistry and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000Immunohistochemistry (Paraffin): 1:100.
• Antigen retrieval in citrate buffer required
• Immunofluorescence (IC): 1:50; Requires methanol permeabilization
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.