The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation.
PA700, PA28 and PA200 are three major protein complexes that function as activators of the 20S proteasome.
There are three evolutionary conserved subunits of PA28; PA28alpha (PSME1), PA28beta (PSME2) and PA28gamma (PSME3) (1,2).
PA28alpha and PA28beta form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s).
The PA28alpha/beta complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptide from foreign proteins (2).
PA28gamma exists in the form of a homoheptamer and is mainly located in the nucleus.
PA28gamma complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation (3,4).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Detects endogenous levels of total PA28 alpha protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hm Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Lys13 of human PA28 alpha
