Several protein-protein interactions are essential to membrane fusion during endocytosis.
Interactions among SNARE1 proteins associated with donor membranes and acceptor membranes are required for fusion (1,2).
After membrane fusion, alpha-SNAP, a cytoplasmic adapter protein binds to the SNARE complex.
N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then can associate with the alpha-SNAP/SNARE complex and mediate SNARE diassembly during the membrane fusion process (3,4).
The ATPase activity of NSF induces a conformational change in the alpha-SNAP/SNARE complex, leading to its dissociation from the membrane and membrane fusion as well as recycling of the SNARE complex for subsequent rounds of fusion (3,4).
Applications:Suitable for use in Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human total NSF protein. at 78kDSpecies Crossreactivity: mouse, rat, monkey, hamster.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hm Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Leu524 of human NSF.
