c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements.
The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2).
Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription.
Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress-induced signaling pathways.
The MAP kinase homologue SAPK/JNK binds to the amino-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73.
In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).
c-Jun has also been reported to become phosphorylated at the C-terminal sites Thr239 and Ser243.
The phosphorylation of Ser243 may participate in its downregulation (5).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of total human c-Jun protein only when phosphorylated at Ser243. Species Crossreactivity: mouse, monkey and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues around Ser243 of human c-Jun.
