84-1087-10　Interferon Regulatory Factor 2 (IRF2) 100ul　I7662-79A

特徴

• Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1).
• IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation.
• The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP.
• All IRF proteins share homology in their amino-terminal DNA-binding domains.
• IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).
• Structurally similar to other IRF family members, IRF-2 acts as both a transcription activator and repressor.
• IRF-2 promotes transcription of several genes, including histone H4, the VCAM1 cell adhesion protein, and CIITA, a MHC transactivator protein.
• Conversely, IRF-2 competitively binds to promoter sites utilized by IRF-1, repressing activation by this related transcription factor (3).
• Its activity may be controlled by inducible proteolysis (4).
• IRF-2 has also been associated with increased oncogenic potential (5,6) and lymphocyte development (7,8).
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.