GLI was first identified as a gene amplified in a malignant glioma (1) and can transform primary cells in cooperation with adenovirus E1A (2).
GLI belongs to the Kruppel family of zinc finger proteins and there are three GLI proteins in mammals: GLI1, GLI2 and GLI3 (3).
GLI proteins, similar to their Drosophila homolog Ci (Cubitus interruptus), function as transcription factors activated by the Hedgehog signaling pathway which plays an important role in animal development and is aberrantly activated in many types of cancers (4,5).
GLI1 itself is a transcriptional target of the Hedgehog signaling pathway (6-8) and is used as a marker of Hedgehog signaling activation in cancer research (9,10).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Immunoprecipitation: 1:50Western Blot: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity：Detects endogenous levels of total human GLI1 protein.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues surrounding Gly420 of human GLI1.
