84-1086-91　CLK3 (cdc2-like Kinase 3) 100ul　C5841-60

特徴

• The cdc2-like kinase (CLK) family contains at least four highly conserved isoforms CLK1, CLK2, CLK3 and CLK4 (1,2).
• CLKs are dual specific kinases that have been shown to autophosphorylate on serine, threonine and tyrosine residues and phosphorylate exogenous substrates on serine and threonine residues (2).
• CLK family members can co-exist as full-length catalytically active forms and an alternatively-spliced truncated inactive form (1).
• A family of highly phosphorylated proteins, so called serine- and arginine-rich (SR) proteins, have been shown to be phosphorylated by CLKs (3-5).
• SR proteins are splicing factors that regulate the assembly of the spliceosome, a macromolecular complex where RNA splicing occurs in the nucleus, and selection of splice sites.
• Thus, CLKs may play important roles in regulating RNA splicing.
• CLK3 is abundantly expressed in the testis and, similar to other family members, has been implicated in regulating RNA splicing (6-8).
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunofluorescence (IF-IC): 1:25Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.