Clathrin-coated pits, intracellular transport vesicles involved in endocytosis and other membrane trafficking processes, are multi-component units.
(1,2).
Clathrin, the most abundant protein in these vesicles, is formed of a basic assembly unit called a triskelion composed of three clathrin heavy chains and three clathrin light chains.
Together with other coat components such as AP2, epsin and EPS15, triskelia are targeted to appropriate membrane fusion sites, where subsequent invagination, cargo protein uptake and vesicle internalization occurs (3,4).
Applications:Suitable for use in Western Blot, Immunofluorescence/ Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunofluorescence / Immunocytochemistry: 1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Clathrin protein at 190kD. Species Crossreactivity: mouse, rat, monkey, bovine.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Bo Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Pro1663 of human Clathrin conjugated to KLH. Species sequence homology: porcine
