Cathepsin D is a ubituitiously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1).
It is synthesized as an inactive 43kD preprocathepsin D that is cleaved and glycosylated to form a 46kD procathepsin D and then further cleaved to produce 28kD and 15kD subunits (heavy and light chains, respectively) (2).
Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3).
Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularily in breast cancer (4-9).
Applications:Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry (Paraffin): 1:200Flow Cytometry: 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human preprocathepsin D, procathepsin D and the heavy chain subunit of mature cathepsin D. Species Crossreactivity: monkey
Isotype：IgG
Calc Applications Abbrev：FC IHC WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to residues with the heavy chain subunit of human Cathepsin D.
