Butyrate response factor 1 (BRF1; also known as EGF response factor 1 [ERF1], TIS11B, ZFP36L1) and butyrate response factor 2 (BRF2; also known as EGF response factor 2 [ERF2], TIS11D, ZFP36L2) both belong to the TIS11 family of CCCH zinc-finger proteins.
This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (ARE) found in the 3’-untranslated regions of mRNAs and promote de-adenylation and rapid degradation by the exosome.
These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors and cell cycle regulators, including GM-CSF, TNF alpha, IL-2, IL-3 and IL-6.
Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation.
Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of BRF1 at Ser92, which results in binding by 14-3-3 protein and inactivation of BRF1.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunofluorescence (IF-IC): 1:25Flow Cytometry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human BRF1 and BRF2 proteins. Species Crossreactivity: mouse, rat and monkey.
Isotype：IgG
Calc Applications Abbrev：E FC IF WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminus of human BRF1 protein. MW: 40-50kD; BRF1, 62kD; BRF2. Species Sequence Homology: bovine, chicken, 100%
