84-1086-86　Bim (Bod, Bcl2l11, BCL2-like 11 Apoptosis Facilitator, Bcl2 Interacting Mediator of Cell Death) 100ul　B1090-01D

特徴

• Bim/Bod is a pro-apoptotic protein belonging to the -BH3-only+ group of Bcl-2 family members (that includes Bad, Bid, Bik, Hrk and Noxa) containing a BH3 domain but lacking other conserved domains, BH1 or BH2 (1,2).
• Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family.
• Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1 and BHRF-1 (1,2).
• Particular functions for Bim have been described in the regulation of apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated (3-6).
• Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL and BimS (1).
• The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis.
• The other isoforms, BimEL and BimL, may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7).
• Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8,9).
• Environmental stress triggers Bim phosphorylation by JNK, resulting in dissociation with the dynein complex and increased apoptotic activity.
• Applications:Suitable for use in Western Blot, Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.