84-1086-84　ATPase alpha 1, Na, K 100ul　A4000-52B

特徴

• The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family.
• This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients.
• It is composed of a catalytic alpha subunit and a beta subunit.
• Several phosphorylation sites have been identified for the alpha1 subunit.
• Tyr10 is phosphorylated by an as yet undetermined kinase, Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA.
• All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase.
• Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule.
• Na,K-ATPase is also involved in other signal transduction pathways.
• Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the alpha subunit.
• Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.