The sequence-specific transcription factor activator protein 2 beta (AP-2 beta) is required for normal kidney development (1).
AP-2 beta knockout mice die 1-2 days after birth due to polycystic kidney disease (1).
Massive apoptosis occured during kidney deveopment at the end of embryogenesis in these mice (1).
Overexpressed AP-2 beta has been to found to suppress c-myc-induced apoptosis, indicating a role of this transcription factor in cell survival (1).
In addition, overexpression of AP-2 beta is shown to be related to impaired insulin signaling in adipocytes, and is therefore proposed to be a candidate gene that may relate to obesity (2).
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Immunofluorescence (IF-IC): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of total human AP-2 beta protein at 49kD. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from the sequence of human AP-2 beta.
