The sequence-specific transcription factor activator protein 2 alpha (AP-2 alpha) is required for normal growth and morphogenesis during mammalian development (1,2).
Decreased or loss of AP-2 alpha expression has been observed in many different types of human cancers including breast cancer (3,4), ovarian cancer (5), melanoma (6) and prostate cancer (7).
These findings suggest that AP-2alpha expression plays a crucial role in tumorigenicity.
Studies have also shown that p53 overexpression in human breast carcinoma cells induces the level of AP-2 alpha expression.
Furthermore, p53 binds to the cis-element in the AP-2alpha promoter, suggesting that AP-2 alpha is a target of p53.
AP-2 alpha may mediate the effect of p53 to inhibit cell proliferation (8).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of total human AP-2 alpha protein. Species Crossreactivity: mouse, rat and monkey.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide derived from a sequence of human AP-2 alpha (KLH).
