AML1 (also known as Runx1, CBFA2 and PEBP2aB) is a member of the CBF (core binding factor) family of transcription factors (1,2).
It is required for normal development of all hematopoietic lineages (3,4,5).
AML1 forms a heterodimeric DNA binding complex with its partner protein CBFb and regulates the expression of cellular genes by binding to promoter and enhancer elements.
AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21)TEL-AML and t(8;21) AML-M2 (6).
Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an ERK-dependent manner (7,8)
Applications:Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of human AML1 protein only when phosphorylated on Ser249.
Isotype：IgG
Calc Applications Abbrev：E FC IF IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids around Ser249 of human AML1.
