Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation.
It is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction.
TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development.
Its receptor TSLP-R will bind TSLP in a low-affinity fashion in transfected cells; cotransfection with IL-7R-alpha cDNA results in high-affinity binding and a functional heteromeric complex.
This heteromeric receptor requires stat5 for TSLP-mediated signal transduction and is inhibited by SOCS-1.
Despite its predicted molecular weight, TSLP-R often migrates at a higher molecular weight in SDS-PAGE.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human liver tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Thymic Stromal Lymphopoietin Protein Receptor.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to19aa near the center of human Thymic Stromal Lymphopoietin Protein Receptor (GenBank accession: Q9HC73).
