In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++- release-activated Ca++ (CRAC) channels (reviewed in 1).
These channels are made up of the pore-forming subunit ORAI1 (2) and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store (3,4).
A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play acoordinated role in controlling Ca++ entry (5).
At least three isoforms of STIM2 are known to exist.
This STIM2 antibody is predicted to have no cross-reactivity to STIM1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1 ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:A-20 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of gylcerol to 40%.
Specificity：Recognizes human STIM2. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide, 18aa from near the carboxy terminus of human STIM2.
