84-1086-70　［取扱停止］Stromal Interaction Molecule 1 (STIM1) 100ug　S7975-88

特徴

• In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++- release-activated Ca++ (CRAC) channels (reviewed in 1).
• These channels are made up of the pore-forming subunit ORAI1 (2) and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store (3,4).
• A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play acoordinated role in controlling Ca++ entry (5).
• At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form.
• This STIM1 antibody is predictedto have no cross-reactivity to STIM2.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2 ug/ml
• Immunohistochemistry: 2.5ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Mouse thymus tissue lysateHuman spleen tissue lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.