Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1).
T-cell activation is generally self-limited as activated T cells express receptors such as PDCD-1 that mediate inhibitory signals from the APC (2).
PDCD-1 can bind two different but related ligands, PDL-1 and PDL-2.
Upon binding to either of these ligands, signals generated by PDCD1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens (3).
Evidence for this is seen in PDCD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases (4).
Despite its predicted molecular weight, PDCD1 often migrates at higher molecular weight in SDS-PAGE.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/ml.
See P3120-60A for control peptide
Optimal dilutions to be determined by the researcher.
Positive Control:THP-1 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human PDCD1. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：16 amino acid peptide from near the center of human PDCD1 (UniProt # Q15116)
