84-1086-49　［取扱停止］MDA5 (Melanoma Differentiation-Associated Protein 5, Interferon-Induced with Helicase C Domain Protein 1, IFIH1) 100ug　M2772-75

特徴

• The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses (1,2).
• Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection.
• MDA5, a member of the DEAD/DEAH-box RNA helicase family (3), consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1.
• When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-κB (4).
• MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C).
• MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity (5).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/ml.
• Optimal dilutions to be determined by the researcher.
• Recommended Control Peptide:M2772-75A: MDA5, Human, Control Peptide (Melanoma Differentiation-Associated Protein 5, Interferon-Induced with Helicase C Domain Protein 1, IFIH1)
• Recommended Positive Control:Daudi cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.