Programmed cell death regulates a number of biological processes such as normal organism development, tissue homeostasis, and removal of damaged cells.
Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1).
LFG is a recently identified protein that can inhibit the apoptotic signal transduced by the Fas receptor but not from the related tumor necrosis factor-α death signal (2).
In this respect, LFG is functionally similar to the anti-apoptotic proteins FAIM, FLIP and Bcl-xL (3).
LFG, a seven membrane spanning protein, can bind the Fas receptor but does not regulate Fas expression or inhibit binding of FADD to Fas.
LFG is widely distributed, but highly expressed in the hippocampus and other neural tissues.
LFG was also identified as the neural membrane protein 35 (NMP35) (4) and its expression is known to be regulated by the Phosphatidylinositol 3-kinase-Akt/PKB pathway (5).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/ml.
See L2185-01 for control peptide
Optimal dilutions to be determined by the researcher.
Positive Control:EL4 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human LFG. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：16 amino acid peptide from near the amino terminus of human LFG (GenBank accession no. AAF06327)
