KAI1 was initially identified from a T-cell activation study as a four-transmembrane protein that plays an accessory role in T-cell activation (1,2), and was later determined to act as a cancer metastasis suppressor gene (3).
This protein is ubiquitously expressed at moderate to high levels in most tissues, but its expression is downregulated during tumor progression.
The loss of KAI1 and p53 is associated with poor survival for prostate and other cancer patients (4).
Recently, KAI1 was found to interact with DARC, the Duffy antigen for chemokines using a yeast two hybrid screen (5).
It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases.
At least two isoforms of KAI1 are known to exist.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/ml.
See K0003-50A for control peptide
Optimal dilutions to be determined by the researcher.
Positive Control:A549 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human KAI1. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：15 amino acid peptide from near the carboxy terminus of human KAI1 (GenBank accession no. NP_002222)
