Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells.
Disruption of this process has been implicated in a variety of diseases suc h as cancer (reviewed in 1).
Aven is a recently discovered protein that blocks apoptosis induced by Apaf-1 and caspase-9 (2).
It is thought that Aven functions by binding to Bcl-xL, an antiapoptotic member of the Bcl-2 family, and to Apaf-1, possibly interfering with the ability of Apaf-1 to self-associate, suggesting that Aven impedes Apaf-1-mediated caspase activation.
Higher levels of Aven mRNA are seen in patients with acute leukemia than in control patients, suggesting that Aven may be useful as a prognostic indicator in leukemia patients (3).
Despite its predicted molecular weight, Aven often migrates at 55kD in SDS-PAGE.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/ml.
See A4445-11A for control peptide
Optimal dilutions to be determined by the researcher.
Positive Control:Raji cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Aven. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：18 amino acid peptide from near the amino terminus of human Aven (GenBank accession no. NP_065104)
