Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils.
Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation.
In disease state oligomerization, extensive amyloid oligomerization creates plaques in neural tissue that correlates with Alzheimer’s symptomology.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: DirectWestern Blot: 1:500 detected Amyloid Oligomer on 10ug of mouse brain lysates
Immunoprecipitation: 1:1000.
Suggested cell lysis buffer is RIPA.
Suggested capture agent is magnetic beads (Dynabeads).
Known co-precipitatiing polypeptide: Amyloid beta, alpha synuclein oligomers
Immunofluorescence: 1:200 diluted 1:1 in a mixture of blocking solution and PBS (refer to protocol)Immunohistochemistry: 1:1000-1:10,000Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a liquid. No preservative added.
Specificity：Recognizes b-Amyloid, oligomer A11. Recognizes all types of amyloid oligomers. Appears to recognize a peptide backbone epitope that is common to amyloid oligomers, but is not found in native proteins, amyloidogenic monomer or mature amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation.Species Crossreactivity: human, mouse, rat and Eukaryote
