TPO (Thrombopoietin, Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo.
Human TPO is a soluble 18.5kD protein containing 174 amino acid residues which comprises the receptor binding domain of the Mpl-ligand protein.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:800Immunocytochemistry: 1:500 Band at 59kDOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul of 10mg/ml BSA in PBS.
Specificity：Recognizes human Thrombopoietin (aa299-332). Reactivity of human Thrombopoietin, recombinant is ~90%. Does not react to human Erythropoietin, recombinant.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Peptide analog of human thrombopoietin (TPO residues #299-332) attached onto a carrier protein.
