Protein Kinase C eta (PKC eta) is an 80kD member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion.
PKC eta has been shown to be involved in the cell cycle as ectopic expression results in elevated expression of cyclins D and E, and its association with the cyclinE/cdk2/p21 complex inhibits cdk2 kinase activity, leading to G1 arrest.
PKC eta is also involved in mediating FGF and PDGF ability to stimulate IL-6 production, and PKC eta modulation of ERK and JNK signaling pathways.
The activation loop threonine (threonine 512 in PKC eta) of novel PKCs is phosphorylated by phosphoinositide dependent kinase-1 (PDK1) and is necessary for the autophosphorylation of threonine 655 in the carboxy-terminus of PKC eta, a step that may play a role in mediating PKC eta signaling events.
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Indirect Immunofluorescence: Specifically stains fixed cultured cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitution with 100ul PBS, 10mg/ml BSA.
Specificity：Recognizes mouse Protein Kinase C eta (aa676-683). Crossreactivity: Protein Kinase C delta (aa662-673): < 0.01%. Does not react to Protein Kinase C alpha (aa664-672), Protein Kinase C beta1 (aa662-671), Protein Kinase C beta2 (aa660-673), Protein Kinase C gamma (aa681-689), Protein Kinase C epsilon (aa728-737), Protein Kinase C xi (aa480-492), Protein Kinase C theta (aa700-706), human ACTH (aa1-39), human ANP, human Calcitonin, Somatostatin 28 or Vasoactive Intestinal Peptide.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to aa673-683 of mouse Protein Kinase C eta covalently attached onto a carrier protein.
