Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitution with 0.1ml of 10mg/ml BSA in PBS.
Specificity：Recognizes Protein Kinase alpha [664-672]. Does not react to Protein Kinase C beta1 [661-671], Protein Kinase C beta2 [660-673], Protein Kinase C gamma [681-689], Protein Kinase C delta [662-673], Protein Kinase C epsilon [728-737], Protein Kinase C xi [480-492], Protein Kinase C eta, Protein Kinase C theta, ACTH (human, 1-39), ANP (human), Calcitonin (human), Somatostatin 28 and Vasoactive Intestinal Peptide. Has been found to stain specifically PKC alpha in Western immunoblots of whole rat brain homogenates and to stain specifically intracellular regions of fixed cultured cells by indirect immunofluorescence.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic Protein Kinase C alpha [664-672] covalently attached onto a carrier protein.
