Polypeptide hormones secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium and promote renal tubular reabsorption of calcium and increased renal excretion of phosphates.
It is a major regulator of bone metabolism.
Secretion of parathyroid hormone increases when the level of calcium in the extracellular fluid is low.
Its action is opposed by calcitonin.
Parathyroid hormone (PTH), or parathormone, is secreted by the parathyroid glands as a polypeptide containing 84 amino acids.
It acts to increase the concentration of calcium (Ca2+) in the blood, whereas calcitonin (a hormone produced by the parafollicular cells (C cells) of the thyroid gland) acts to decrease calcium concentration.
PTH acts to increase the concentration of calcium in the blood by acting upon parathyroid hormone receptor in three parts of the body.
PTH half-life is ~4 minutes.
PTH enhances the release of calcium from the large reservoir contained in the bones.
Bone resorption is the normal destruction of bone by osteoclasts, which are indirectly stimulated by PTH.
Stimulation is indirect since osteoclasts do not have a receptor for PTH; rather, PTH binds to osteoblasts, the cells responsible for creating bone.
Binding stimulates osteoblasts to increase their expression of RANKL, which can bind to osteoclast precursors containing RANK, a receptor for RANKL.
The binding of RANKL to RANK stimulates these precursors to fuse, forming new osteoclasts which ultimately enhances the resorption of bone.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS, BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 10mg/ml BSA.
Specificity：Recognizes human Parathyroid Hormone (PTH), aa39-84. Reactivity of human PTH, aa1-84 is 90%. Does not react with human PTH (aa1-34), PTHrp (aa1-35), ANP, human ACTH (aa1-39) or VIP.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa39-84 of human Parathyroid Hormone (PTH) covalently attached onto a carrier protein.
