Polypeptide hormones secreted by the parathyroid glands, which promote release of calcium from bone to extracellular fluid by activating osteoblasts and inhibiting osteoclasts, indirectly promote increased intestinal absorption of calcium and promote renal tubular reabsorption of calcium and increased renal excretion of phosphates.
It is a major regulator of bone metabolism.
Secretion of parathyroid hormone increases when the level of calcium in the extracellular fluid is low.
Its action is opposed by calcitonin.
Parathyroid hormone (PTH), or parathormone, is secreted by the parathyroid glands as a polypeptide containing 84 amino acids.
It acts to increase the concentration of calcium (Ca2+) in the blood, whereas calcitonin (a hormone produced by the parafollicular cells (C cells) of the thyroid gland) acts to decrease calcium concentration.
PTH acts to increase the concentration of calcium in the blood by acting upon parathyroid hormone receptor in three parts of the body.
PTH half-life is approximately 4 minutes.
Applications:Suitable for use in RIA, ELISA and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Immunoprecipitation: This antiserum has been found to immunoprecipitate quantitatively at dilutions of 1:400 by RIA techniques using goat anti-rabbit IgG antibody and carrier rabbit IgG for double antibody precipitation and using magnetic beads coated with goat anti-rabbit IgG antibody.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS, 10mg/ml BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul of sterile PBS, 10mg/ml BSA.
Specificity：Recognizes PTH (1-34) (human) and PTH (1-84) (human). The reactivity of PTH (1-84) (bovine) is 80% and PTHrp (1-35) ia <0.1%. Does not react to Atrial natriuretic peptide, Calcitonin (human), CGRP (human) or VIP. Specific for hPTH (1-34) and is suitable for the immunoprecipitation of hPTH (1-34) and intact hPTH.
Isotype：IgG
Calc Applications Abbrev：E IP RIA
Calc Crossreactivity：Hu
Immunogen：Human PTH (1-34) covalently attached onto a carrier protein.
