84-1085-97　Osteocalcin, aa40-49 (Bone-Gla-Protein, BGP) 100ul　O8060-14J

特徴

• Osteocalcin, also known as Bone-Gla-Protein (BGP), is a single chain (Mr=5800) vitamin K dependent protein produced by osteoblasts.
• It is found in high concentrations in bone.
• Post-translational modification by a vitamin K dependent carboxylase produces three g-carboxyglutamic acid residues at positions 17, 21 and 24.
• The mature protein contains 49 amino acids and a single intrachain disulfide bond joining Cys23 to Cys29.
• The secondary structure is highly calcium dependent and contains 14% alpha-helix, 20% beta-sheet and 67% random form in the presence of calcium, and 1% alpha-helix, 20% beta-sheet and 79% random form in the absence of calcium.
• BGP binds to phospholipid vesicles in the presence of calcium ions (Kd=6 x 10e-6 M).
• BGP also binds hydroxyapatite and is an efficient inhibitor of hydroxyapatite seeded crystal growth, suggesting a regulatory role in bone mineralization.
• Although the fundamental role of BGP in vivo remains elusive, in vitro experimentation suggests roles in both bone resorption and bone formation.
• BGP has been shown to function as a chemo-attractant in an in vitro macrophage mediated bone resorption system.
• The serum concentration of BGP has been linked to bone formation rates, and BGP levels are currently used in research assessment of osteoporosis and other metabolic bone disease states.
• Applications:Suitable for use in ELISA and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS, 10mg/ml BSA..
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.