The function of the delta-opioid receptors has not been determined.
However, recent studies in rodents have linked delta-opioid receptor antagonists with prevention of morphine tolerance and dependence without relinquishing mu-opioid antinociception.
In general, opioids modulate numerous central and peripheral processes, including pain perception, neuroendocrine secretion and the immune response.
The opioid signal is transduced from receptors through G proteins to various different effectors.
Subsequent to G protein activation, several effectors are known to orchestrate the opioid signal.
For example, activation of opioid receptors increases phosphatidylinositol turnover, activates K+ channels and reduces adenylyl cyclase and Ca++ channel activities.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: ≥1:8 with TBS/Tween-20, 1% normal goat serum
Optimal dilutions to be determined by the researcher.
Included Control Peptide:O7030-15D1: Opioid Receptor, delta (360-372) Reconstitute with 600ul of reconstituted antibody.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile TBS/Tween-20 containing 1% normal goat serum.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：5ml
Host：rabbit
Grade：Purified
Purity：Purified by precipitation, dialysis and chromatography.
Form：Supplied as a lyophilized powder. Reconstitution with 5.0 ml of TBS/Tween 20 containing 1% normal goat serum.
Specificity：Recognizes delta Opioid Receptor (360-372) and the reactivity of delta Opioid Receptor is ~60%. Does not react to Mu Opioid Receptor and Kappa Opioid Receptor. Has been found to stain specifically the delta opioid receptor in Western immunoblots of whole rat brain homogenates.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Peptide analog of the carboxyl terminal of the delta opioid receptor covalently attached onto a carrier protein.
